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skeletal muscle fiber types  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank skeletal muscle fiber types
    Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and <t>skeletal</t> muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of <t>muscle</t> tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different <t>fiber</t> <t>types</t> in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).
    Skeletal Muscle Fiber Types, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging"

    Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2025.1667437

    Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and skeletal muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of muscle tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different fiber types in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).
    Figure Legend Snippet: Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and skeletal muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of muscle tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different fiber types in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).

    Techniques Used: Muscles

    Inactivation of linc-MYH increases the number of slow-twitch muscle fibers in soleus muscles of geriatric mice. (a) Representative images of muscle fibers in TA and soleus muscles stained with antibodies against different MyHC isoforms. (b) Stacked bar graph showing the proportion of type I, type IIa, type IIb and type IIx fibers in cross-sectioned TA and soleus muscles (n = 4 WT, n = 4 linc-MYH −/− ), ** p < 0.01, **** p < 0.0001. (c) Schematic representation of different muscle fiber types and metabolic characteristics. Inactivation of linc-MYH induces a shift toward oxidative muscle fibers.
    Figure Legend Snippet: Inactivation of linc-MYH increases the number of slow-twitch muscle fibers in soleus muscles of geriatric mice. (a) Representative images of muscle fibers in TA and soleus muscles stained with antibodies against different MyHC isoforms. (b) Stacked bar graph showing the proportion of type I, type IIa, type IIb and type IIx fibers in cross-sectioned TA and soleus muscles (n = 4 WT, n = 4 linc-MYH −/− ), ** p < 0.01, **** p < 0.0001. (c) Schematic representation of different muscle fiber types and metabolic characteristics. Inactivation of linc-MYH induces a shift toward oxidative muscle fibers.

    Techniques Used: Muscles, Staining

    Related Articles

    Muscles:

    Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging
    Article Snippet: Finally, sections were embedded using Fluoromount W (21634.01, Serva).Finally, sections were embedded using Fluoromount W (21634.01, Serva).. Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).. Secondary antibodies were goat anti mouse IgG Fcγ 2b DyLight405 (Jackson Immuno, 115-475-207), goat anti mouse IgG Fcγ 1 Alexa 488 (Jackson Immuno, 115-545-205), and goat anti mouse IgM Alexa594 (Jackson Immuno, 115-85-075).Secondary antibodies were goat anti mouse IgG Fcγ 2b DyLight405 (Jackson Immuno, 115-475-207), goat anti mouse IgG Fcγ 1 Alexa 488 (Jackson Immuno, 115-545-205), and goat anti mouse IgM Alexa594 (Jackson Immuno, 115-85-075).

    Staining:

    Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging
    Article Snippet: Finally, sections were embedded using Fluoromount W (21634.01, Serva).Finally, sections were embedded using Fluoromount W (21634.01, Serva).. Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).. Secondary antibodies were goat anti mouse IgG Fcγ 2b DyLight405 (Jackson Immuno, 115-475-207), goat anti mouse IgG Fcγ 1 Alexa 488 (Jackson Immuno, 115-545-205), and goat anti mouse IgM Alexa594 (Jackson Immuno, 115-85-075).Secondary antibodies were goat anti mouse IgG Fcγ 2b DyLight405 (Jackson Immuno, 115-475-207), goat anti mouse IgG Fcγ 1 Alexa 488 (Jackson Immuno, 115-545-205), and goat anti mouse IgM Alexa594 (Jackson Immuno, 115-85-075).



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    Edstrom Industries morphological and histochemical types of fiber in mammalian skeletal muscle
    Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and <t>skeletal</t> muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of <t>muscle</t> tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different <t>fiber</t> <t>types</t> in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).
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    Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and skeletal muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of muscle tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different fiber types in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging

    doi: 10.3389/fcell.2025.1667437

    Figure Lengend Snippet: Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and skeletal muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of muscle tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different fiber types in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).

    Article Snippet: Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).

    Techniques: Muscles

    Inactivation of linc-MYH increases the number of slow-twitch muscle fibers in soleus muscles of geriatric mice. (a) Representative images of muscle fibers in TA and soleus muscles stained with antibodies against different MyHC isoforms. (b) Stacked bar graph showing the proportion of type I, type IIa, type IIb and type IIx fibers in cross-sectioned TA and soleus muscles (n = 4 WT, n = 4 linc-MYH −/− ), ** p < 0.01, **** p < 0.0001. (c) Schematic representation of different muscle fiber types and metabolic characteristics. Inactivation of linc-MYH induces a shift toward oxidative muscle fibers.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging

    doi: 10.3389/fcell.2025.1667437

    Figure Lengend Snippet: Inactivation of linc-MYH increases the number of slow-twitch muscle fibers in soleus muscles of geriatric mice. (a) Representative images of muscle fibers in TA and soleus muscles stained with antibodies against different MyHC isoforms. (b) Stacked bar graph showing the proportion of type I, type IIa, type IIb and type IIx fibers in cross-sectioned TA and soleus muscles (n = 4 WT, n = 4 linc-MYH −/− ), ** p < 0.01, **** p < 0.0001. (c) Schematic representation of different muscle fiber types and metabolic characteristics. Inactivation of linc-MYH induces a shift toward oxidative muscle fibers.

    Article Snippet: Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).

    Techniques: Muscles, Staining